New technique for in-cell distance determination
Researchers from the University of Konstanz, Bielefeld University and ETH Zurich demonstrate for the first time that the pulsed EPR technique RIDME (relaxation-induced dipolar modulation enhancement) can be used for in-cell distance determination in biomacromolecules. Applied within the cell, RIDME improves significantly on conventional double electron–electron resonance (DEER) measurements.
In a joint paper which has just been published in The Journal of Physical Chemistry Letters, researchers from the University of Konstanz, Bielefeld University and ETH Zurich demonstrate for the first time that the electron paramagnetic resonance (EPR) technique RIDME (relaxation-induced dipolar modulation enhancement) can be applied to determine distances between gadolinium(III)-based spin labels in cells. In-cell distance determination by electron paramagnetic resonance (EPR) reveals essential structural information about biomacromolecules, including their conformation as well as folding and unfolding processes. Read more...